Ahmad Bassiouny
Alexandria University, Egypt.
Title: Neuroprotective evaluation of extract of ginger (Zingiber officinale) root in MPTP-induced toxicity in different brain areas male mouse C57/BL/6 models.
Biography
Biography: Ahmad Bassiouny
Abstract
Parkinson's disease is a progressive disorder of the nervous system that affects motor function in basal ganglia. The aim of this study was to examine the effects of ginger extract on neuroinflammatory-induced damage of dopaminergic (DA) neurons in Parkinson's disease (PD) mouse C57/BL/6 models. Animals were injected intraperitoneally (IP) with a total cumulative dose of 150 mg/kg MPTP. The levels of dopamine were determined by HPLC. Chronic exposure to neurotoxins increase α-synuclein (αS) aggregation concomitant with upregulation of miR-155 and downregulation of miR-7 and -153 and increase in intracellular reactive oxygen species (ROS). Seven days after the last MPTP injection, behavioral testings were performed. The levels of TNF-α, COX-2 and iNO and miR-7, miR-153, miR-155 were analyzed both in Substantia nigra pars compacta (SNpc) and globus pallidus (GP) by real time PCR. Results: Here we show that Ginger extract can alleviate αS-induced toxicity, downregulate miR-155, and upregulate miR-7 and miR-153, reduce ROS levels and protect cells against apoptosis. It significantly increased the level of dopamine in GP and striatum and suppressed TNF-α and NO levels. In C57/BL mice, treatment with Ginger extract reversed MPTP-induced changes in motor coordination and bradykinesia. Moreover, Ginger extract significantly inhibited the MPTP-induced microglial activation and increases in the levels of TNF-α, NO, iNOS, and COX-2 in both SNpc and GP. It upregulated the level of miR-153 and miR-7 indicating a protective effect. Conclusion: Our results may indicate that miR155 has a possible central role in the inflammatory response to αS and αS-related neurodegeneration. These effects are at least in part due to a direct role of miR-155 on the microglial response to αS. Our findings implicate miR-155, miR-153 and miR-7 are potential therapeutic targets for regulating the inflammatory response in PD. Ginger extract exerts neuroprotective effects on DA neurons in in vivo PD model.